The rules aim to eliminate deforestation from the supply chains of a range of everyday items sold in Europe.
It will apply to soy, beef, palm oil, wood, cocoa, coffee, rubber, charcoal, and derived products including leather, chocolate and furniture.
Indonesia and Malaysia, the world's largest palm oil exporters, have accused the EU of blocking market access for their palm oil.
The EU is the world's third-largest palm oil importer.
The EU Parliament approved a deal on the rules, which was agreed by EU negotiators last year.