Under the Republican map, only one of the state's seven congressional districts is majority Black, even though Black residents make up more than a quarter of the state's population.
The office of Attorney General Steve Marshall, a Republican, said the state would appeal to the U.S. Supreme Court.
The plaintiffs who challenged Alabama's map, including the state chapter of the NAACP, in a joint statement said: "Alabama openly admits its intention to defy the law and the U.S. Supreme Court.
The Supreme Court also in June allowed a challenge to Louisiana's congressional map to advance.
A federal court has ordered lawmakers to draw a second majority-Black district, and a U.S. appeals court is set to review the case next month.
Persons:
Michael A, McCoy, Terri Sewell, Steve Marshall, Ron DeSantis, Joseph Ax, Colleen Jenkins, Bill Berkrot
Organizations:
Democratic, Selma Fire, REUTERS, Republican, Democratic U.S, U.S, Supreme, NAACP, Republicans, U.S . House, Representatives, Thomson
Locations:
Selma, Selma , Alabama, U.S, Birmingham, Alabama, Florida, Black