The protests are the latest face-off between Santa Cruz, led by right-wing Governor Luis Fernando Camacho, and leftist President Luis Arce's government.
Camacho has maintained his innocence and called his arrest and transport to La Paz, the country's capital, a kidnapping.
The governor became a face for the right-wing opposition movement as a civic leader who called for leftist Morales to step down in 2019.
The government has not said how it will respond to Friday's roadblocks, though some military forces were spread throughout Santa Cruz late Thursday.
Reporting by Nadia Arce in Santa Cruz and Santiago Limachi in La Paz; Writing by Kylie Madry; Editing by Chizu Nomiyama and Leslie AdlerOur Standards: The Thomson Reuters Trust Principles.