Thompson-Herah's agency, Andi Sports Management, said the split was a result of a "breakdown in negotiations on a compensation package" sought by Osbourne.
"Collectively, we had no choice but to seek the services of another coach.
The agency said the process of finding the Jamaican sprinter a new coach had begun.
"With the 2024 Summer Olympics in Paris, France fast approaching, Mrs Thompson-Herah is fully focused on her preparations for the season and the defense of her Olympic titles," it said.
Thompson-Herah, 31, became the first woman to win back-to-back Olympic sprint doubles when she retained her 100m and 200m titles in Tokyo in 2021.
Persons:
Elaine Thompson, Herah, Shanikie Osbourne, Thompson, Osbourne, Mrs Thompson, Florence Griffith, Joyner, Robert Birsel
Organizations:
Andi Sports Management, Thomson
Locations:
Paris, France, Tokyo