Sinead McSweeney, global vice president for public policy, has left Twitter, according to two sources.
The layoffs and departures of a high-ranking leader comes as regulators around the globe question Twitter's content moderation work and protection of user data after Musk cut the staff from more than 7,000 to under 2,000.
The public policy team is responsible for interacting with lawmakers and civil society on issues including free speech, privacy and online safety.
Nick Pickles, senior director for global public policy strategy, has taken over McSweeney's role, the two sources said.
One source told Reuters that half of the 30 remaining members of Twitter's public policy team were cut on Wednesday, implying 15 people were laid off.