A judge ordered Walmart to pay the worker $420,000 and rehire her, citing disability discrimination.
Walmart requested a new trial, but that request has now been denied.
In March, Walmart was also ordered to pay Spaeth a further $119,660, including back pay, and to rehire her.
"Wal-Mart failed to accommodate Ms. Spaeth, unlawfully disciplined her, terminated her employment, and failed to rehire her," the EEOC wrote.
William C. Griesbach, district judge for the Eastern District of Wisconsin, denied Walmart's request for a new trial and to remit damages on Monday.